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Genotyping was performed by PCR with the following primer sets, resolved by electrophoresis on 0.5X TBE agarose gels (1.02.0%): (1) For Cacna1a (Ca V 2.1) KO, a single multiplexed PCR was performed for each animal with four primers targeting exon 4 of Cacna1a , deleted in the mutant line: 5-CGTTCCTTGCGCACGTGTGCT C-3 5-GGGATCATCGCCTTCATGATTGACTTCAGGACGA CT-3

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Graf and Kastin documented that DSIP affects not just sleep itself but the neuroendocrine systems that govern sleep including ACTH, growth hormone release patterns, and circadian oscillators (Graf & Kastin, 1984)