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In the previous study, we had successfully generated FGF5 -knockout heterozygous sheep using the CRISPR/Cas9 system by direct one-step cytoplasmic injection of Cas9 mRNA and sgRNA into zygotes, and the relationship between FGF5 gene and wool length in sheep was proved 22

10.1016/j.mito.2019.10.007 29

coli expression system (Zorbas et al., 2015

Izbicka E, Lawrence R, Cerna C, Von Hoff DD and Sanderson PE
